In vitro studies of antimicrobial activity of (curcuma longa l.) rhizomes against helicobacter pylori

Abstract

Objective Studies on curcumin (Curcuma longa) powder have shown several biological actions such as antibacterial activity. Some microorganisms have an effect on human health by causing diseases, and one of these microorganisms is Helicobacter pylori. H. pylori has attracted great attention as a major cause of gastritis and peptic ulcer diseases. It is the first bacterium to be classified as a group I carcinogen by the International Agency for Research on Cancer. Because of increasing bacterial resistant strains, undesirable side effects, the cost of the antibiotic regimens, and other factors contributing to ineffectiveness, there is an urgent need to develop new treatment strategies for H. pylori infection. This project considered as an explorer study for the inhibitory effect of turmeric (Curcuma longa) powder against H. pylori.
Methods Curcumin extracted with dimethylsulfoxide (DMSO). The antibacterial activity of curcumin was determined by well diffusion method using Brain Heart Infusion agar (BHI) previously spread with 24 h old culture of the H. pylori organisms.
Result Curcumin exhibited good antibacterial activity against H. pylori (zone of inhibition in mm). The extract was effective in inhibiting the bacteria with zone of inhibition, 7.7 mm compared with the inhibition zone of Amoxicillin 8.5 mm.
Conclusion The results suggest that the curcumin exhibited good activity against H. pylori suggesting its potential as an alternative therapy, and further research may be needed to understand the in-depth mechanisms of an effective antibacterial material for eradication of this bacteria.
Keywords Curcuma longa L., H. pylori, antibacterial activity