The biological activities of seeds extracts for fenugreek and black cumin and its inhibitory influences towards some pathogens
Abstract
Objective: The aim of this study is antibacterial activity of Fenugreek and Black cumin against some pathogene.
Methods:The bioactive components of the seeds of Fenugreek( Trigonella Foenum-groecum L ) and Black cumin (Nigella sativa) are scrutinized by GC/MS technique, beta.-D-Glucopyranoside, methyl(35.60%), alpha.-d-Mannofuranoside, methyl(28.31), and Diethyl Phthalate(5.94%) such components found in methanolic fenugreek seed extract. On the other side, the major components of methanolic black seed extract are 9,12-Octadecadienoic acid (Z,Z)-(50.16%), l-(+)-Ascorbic acid 2,6-dihexadecanoate(10.75%), Propyleneglycol monoleate (9.61%), Diethyl Phthalate(3.32%), and Phenol, 2-methyl-5-(1-methylethyl)-(0.17%). In this study, four types of pathogenic bacteria are involved Streptococcus agalactiae, Proteus mirabillis, Enterococcus cloacae and Escherichia coli, that subjected to antibacterial tests using methanolic fenugreek and black cumin seed extracts at concentrations (50,100)mg/ml.
Results: The highest percentage inhibiting of methanolic fenugreek seed extract versus Streptococcus agalactiae is 13.5mm at each conc. (50,100)mg/ml . Furthermore, Escherichia coli is the lowest effectiveness of black cumin seed extract which reached (9.5,7.5 )mm at conc. (50,100)mg/ml, comparison with control.
Conclusions: In this study , the presence of bioactive compounds as beta.-D-Glucopyranoside, alpha.-d-Mannofuranoside, methyl, Diethyl Phthalate, ,12-Octadecadienoic acid (Z,Z)-,and l-(+)-Ascorbic acid 2,6-dihexadecanoate in seeds of fenugreek and black cumin which is associated with inhibiting effect against some pathogenic bacteria. The variations in chemical compounds model can be used to distinguish among medicinal plants.
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