The biological activities of seeds extracts for fenugreek and black cumin and its inhibitory influences towards some pathogens

Authors

  • Khanssa A. Shaheed Department of Biology, College of science , University of Kerbala, Kerbala, Iraq
  • Manal A. Alsirraj Department of Animal productions, College of Agriculture , University of Kerbala, Kerbala, Iraq
  • Shatha A. Allaith Department of Field Crop, College of Agriculture , University of Kerbala, Kerbala, Iraq
  • Noor A. Noori Department of Field Crop, College of Agriculture , University of Kerbala, Kerbala, Iraq
  • Mohammed H. Obaid Department of Horticulture, College of Agriculture , University of Kerbala, Kerbala, Iraq
  • Zeina M. Mouhsan Department of Plant Protection, College of Agriculture , University of Kerbala, Kerbala, Iraq

Abstract

Objective: The aim of this study is antibacterial activity of Fenugreek and Black cumin against some pathogene.

Methods:The bioactive components of the seeds of Fenugreek( Trigonella Foenum-groecum L ) and Black cumin (Nigella sativa) are scrutinized by GC/MS technique, beta.-D-Glucopyranoside, methyl(35.60%), alpha.-d-Mannofuranoside, methyl(28.31), and Diethyl Phthalate(5.94%) such components found in methanolic fenugreek seed extract. On the other side, the major components of methanolic black seed extract are 9,12-Octadecadienoic acid (Z,Z)-(50.16%), l-(+)-Ascorbic acid 2,6-dihexadecanoate(10.75%), Propyleneglycol monoleate (9.61%), Diethyl Phthalate(3.32%), and Phenol, 2-methyl-5-(1-methylethyl)-(0.17%). In this study, four types of pathogenic bacteria are involved Streptococcus agalactiae, Proteus mirabillis, Enterococcus cloacae and Escherichia coli, that subjected to antibacterial tests using methanolic fenugreek and black cumin seed extracts at concentrations (50,100)mg/ml.

Results: The highest percentage inhibiting of methanolic fenugreek seed extract versus Streptococcus agalactiae is 13.5mm at each conc. (50,100)mg/ml . Furthermore, Escherichia coli is the lowest effectiveness of black cumin seed extract which reached (9.5,7.5 )mm at conc. (50,100)mg/ml, comparison with control.

Conclusions: In this study , the presence of bioactive compounds as beta.-D-Glucopyranoside, alpha.-d-Mannofuranoside, methyl, Diethyl Phthalate, ,12-Octadecadienoic acid (Z,Z)-,and l-(+)-Ascorbic acid 2,6-dihexadecanoate in seeds of fenugreek and black cumin which is associated with inhibiting effect against some pathogenic bacteria. The variations in chemical compounds  model can be used to distinguish among medicinal plants.

 

References

1. Snehlata, H. S. and Dande, P. R., “Fenugreek (Trigonella foenum-graecum L.): An Overview,” Int. J. Curr. Pharm. Rev. Res., vol. 2, no. 4, pp. 170–187, 2012.
2. Mohamed ,H. A. El-Sayed ,I. H. and Moawad, M. “Protective effect of Nigella sativia seeds against dimethylaminoazobenzene ( DAB ) induced liver carcinogenesis,” Cancer Biology;1(1):43-50,2011.
3. Goncalves, S., Gomes, D., Costa , P. and Romano, A. The phenolic content and antioxidant activity of infusions from Mediterranean medicinal plants. Industrial Crops and Products 43,465-471,2013.
4. Khoobchandani, M., Ogeswi, B. K., Ganesh,N., Srivastava, M.M., Gabbanini,S., Matera, R., Jori,R. and Valgimigli, L.Antimicrobial prosperities and analytical profile of traditional Eruca sativa seed oil : Comparison with various aerial and root plant extracts .Food Chemistry 120(1): 217-224,2010.
5. Salem , ML. Immunomodulatry and therapeutic properities of Nigella sativa L. Seed .Int.Immunpharamacol ,5:1749-1770,2005.
6. Denys J. Charles.Antioxidant Properties of Spices, Herbs and Other Sources. Springer New York Heidelberg Dordrecht London.295-427,2013.
7. Tesarova H, Svobodova B, Kokoska L, Marsik P, Pribylova M, Landa P, Vadlejch J Determination of oxygen radical absorbance capacity of black cumin (Nigella sativa) seed quinone compounds. Nat Prod Commun 6(2):213–216,2011.
8. Rajendra, G. Kyong-Su, K. Bioactive volatile compounds of three medicinal plants from Nepal. Journal of Science , Engineering and Technology.8(1),51-62,2012.
9. Chaudhary N. , Husain Sh. S., Ali M. Chemical composition and antimicrobial activity of volatile oil of the seeds of Cuminum cyminum L.World journal of pharmacy and pharmaceutical science. 3(7):1428-1441,2014.
10.Dua, A. Garg, G. Mahajan, R. Polyphenols, flavonoids and antimicrobial properties of methanolic extract of fennel(Foeniculum vulgare Miller). European journal of experimental biology. (4):203-208,2013.
11. Allaithy, S. A. M.2017. The chemical compound of cumin and fennel seed extracts against some types of pathogenic bacteria. Iraq Med J;1(1) 1–6.
12. Al-Fekaiki,Dh. F.,Ali. K.A,. Extraction and Diagnosis of Compounds of Phenolic Compounds from thePlanthenna (Lawsoniainermis)and Assess Their Effectiveness as Antioxidants. Thi-Qar J. Agric, Res. , 2 (2)(140-157) 2013.
13. Arora, D. S., Kaur,G. J. Antibacterial activity of some Indian plants.Journal of Natural Medicines. 61( 3);313-317,2007.
14. Speak, M.Compendium of a method for the microbiological examination of food.2th Ed.Washington,D.C.USA.(1984).
15. Panghal, M. Kaushal, V. Yadav, J.P . In vitro antimicrobial activity of ten medicinal plants against clinical isolates of oral cancer cases. Ann Clin Microbiol Antimicrob 10:21,2011.
16. Resan, Rafal Abdul –Hussein. Study off Effect Extraction Methods on Components off Essential Oils for Some Types off Umbelliferae Familly by GC—MS, M.S.c. Thesis in food science . College of Agriculture University of Basra:1-27,2016.
17. Bakathir, H.A. Abbas, N.A. Detection of the antibacterial effect of nigella sativa ground seeds with water. Afr J Tradit Complement Altern Med 8(2):159–164,2011.
18. Abdulhussein,T. M. Shatha, A.Allaithi, Aemen J.M. Zeina, M.M. Evaluation of Inhibitory Efficiency for Some Aqueous and Alcoholic Extracts of Cumin, Fenugreek, Sweet Fennel and Black Cumin in Growth of Escherichia Coli and Staphylococcus Aureus Bacteria. Ibn Al-Haitham J. for Pure & Appl. Sci. 30 (1).279-287,2017.
19. Abdalah, M. E. The study of Antibacterial activity of fenugreek (Trigonellafoenum-graecum) Seeds extract.Iraqi journal of markets research and safety of consumer,3(6),145-155,2011.
20. Mitscher,I.A. Len, R.P. Bathala, M.S. Wu,W.A.and Beal,J.L. Antimicrobial agents from higher plant . lioyid,35(2) :157-166,2011.
21- Idue, M.N. Hatha, A.A. Abirosh, C. Harsha, H. and Vivekanandan, G. Antimicrobial activity of some of the south Indian spices against serotype of Escherichia coli , Salmonella Listeria monocytogens &Aeromonus hydrophila . Brazilian J. of microbiology ,37:153-158,2006.
22. Al-Abdullah ,B.Y. and Waleed, F. J. Production of Functional Drinks From Some Medicinal Herbs and Studying their Microbial Tests and Diagnosis The Chemical Components in them by GC-MS Technique.Journal of Uni. Of Tikrit for Agriculture Sci.15(3);170-183,2015.
23- Hamadii, R.F. Effect of some plant extract on some bacterial isolated causing urinary tract infection (UTI). Anbar .J.3(3),2012.
24. Hasan, N.A. Nawahwi, M.Z. and Malek, H. Antimicrobial activity of Nigella sativa Seed extract .Sains Malysiana,42(2): 143-147,2013.

Downloads

Published

2018-06-26

How to Cite

1.
Shaheed KA, Alsirraj MA, Allaith SA, Noori NA, Obaid MH, Mouhsan ZM. The biological activities of seeds extracts for fenugreek and black cumin and its inhibitory influences towards some pathogens. Iraq Med J [Internet]. 2018 Jun. 26 [cited 2024 Dec. 21];2(2). Available from: https://iraqmedj.org/index.php/imj/article/view/393

Issue

Vol. 2 No. 2 (2018): Spring 2018

Section

Articles