Assessment of GATA3 expression in duodenal biopsies of celiac disease suspected and diagnosed patients
Abstract
Objective In this study we aimed to assess the staining of GATA3 in the duodenal biopsy of celiac disease (CD) patients as step toward improving its diagnosis.
Methods Duodenal biopsied tissues from 50 patients suspected of celiac disease were examined by conventional staining followed by immunohistochemistry using monoclonal antibody GATA3 for tissue sections.
Results The histopathological examination of the samples showed a low GATA3 staining in the duodenal tissue of CD patients.
Conclusion This study concludes that the low staining of GATA3 in the duodenal CD patients indicates the little consequence of Th2 lymphocyte in the disease pathogeneses. This finding is of importance and could be of use in the diagnosis of celiac disease.
References
1. Bao F, Green PH, Bhagat G. An update on celiac disease histopathology and the road ahead. Arch Pathol Lab Med. 2012;136:735–745.
2. Kim SM, Mayassi T, Jabri B. Innate immunity: actuating the gears of celiac disease pathogenesis. Best Pract Res Clin Gastroenterol. 2015;29:425–435.
3. Villanacci V, Ceppa P, Tavani E, Vindigni C, Volta U; Gruppo Italiano Patologi Apparato Digerente (GIPAD), et al. Coeliac disease: the histology report. Dig Liver Dis. 2011;43:S385–S395.
4. Fernández Bañares F, Farré C, Carrasco A, Mariné M, Esteve M. New Tools for the Diagnosis of Celiac Disease. OmniaScience Monographs. 2015.
5. Mustalahti K, Catassi C, Reunanen A, Fabiani E, Heier M, McMillan S, et al. The prevalence of celiac disease in Europe: results of a centralized, international mass screening project. Ann Med. 2010;42:587–595.
6. Al-Hussaini A, Troncone R, Khormi M, AlTuraiki M, Alkhamis W, Alrajhi M, et al. Mass Screening for Celiac Disease Among School-aged Children: Toward Exploring Celiac Iceberg in Saudi Arabia. J Pediatr Gastroenterol Nutr. 2017;65:646–651.
7. Barada K, Bitar A, Mokadem MA, Hashash JG, Green P. Celiac disease in Middle Eastern and North African countries: a new burden? World J Gastroenterol. 2010;16:1449–1457.
8. Barada K, Abu Daya H, Rostami K, Catassi C. Celiac disease in the developing world. Gastrointest Endosc Clin N Am. 2012;22:773–796.
9. Bai JC, Fried M, Corazza GR, Schuppan D, Farthing M, Catassi C, et al. World Gastroenterology Organisation global guidelines on celiac disease. J Clin Gastroenterol. 2013;47:121–126.
10. Kurppa K, Ashorn M, Iltanen S, Koskinen LL, Saavalainen P, Koskinen O, et al. Celiac Disease Wthout Villous Atrophy in Children: A Prospective Study. J Pediatr. 2010;157:373–380.
11. Hudacko R, Kathy Zhou X, Yantiss RK. Immunohistochemical stains for CD3 and CD8 do not improve detection of gluten-sensitive enteropathy in duodenal biopsies. Mod Pathol. 2013;26:1241–1245.
12. Hayday A, Theodoridis E, Ramsburg E, Shires J. Intraepithelial lymphocytes: exploring the Third Way in immunology. Nat Immunol. 2001;2:997–1003.
13. Neutra MR, Mantis NJ, Kraehenbuhl JP. Collaboration of epithelial cells with organized mucosal lymphoid tissues. Nat Immunol. 2001;2:1004–1009.
14. Cheroutre H, Madakamutil L. Acquired and natural memory T cells join forces at the mucosal front line. Nat Rev Immunol. 2004;4:290–300.
15. Abadie V, Discepolo V, Jabri B. Intraepithelial lymphocytes in celiac disease immunopathology. Semin Immunopathol. 2012;34:551–566.
16. Meresse B, Malamut G, Cerf-Bensussan N. Celiac disease: an immunological jigsaw. Immunity. 2012;36:907–919.
17. Abadie V, Jabri B. Immunopathology of Celiac Disease. Mucosal Immunol (Fourth Edition). 2015;2:1551–1572.
18. Cheroutre H, Lambolez F, Mucida D. The light and dark sides of intestinal intraepithelial lymphocytes. Nat Rev Immunol. 2011;11:445–456.
19. Tahoun A, Mahajan S, Paxton E, Malterer G, Donaldson DS, Wang D, et al. Salmonella transforms follicle-associated epithelial cells into M cells to promote intestinal invasion. Cell Host Microbe. 2012;12:645–656.
20. Hoyler T, Connor CA, Kiss EA, Diefenbach A. T-bet and Gata3 in controlling type 1 and type 2 immunity mediated by innate lymphoid cells. Curr Opin Immunol. 2013;25:139–147.
21. Samson SI, Richard O, Tavian M, Ranson T, Vosshenrich CA, Colucci F, et al. GATA-3 promotes maturation, IFN-gamma production, and liver-specific homing of NK cells. Immunity. 2003;19:701–711.
22. Kim PJ, Pai SY, Brigl M, Besra GS, Gumperz J, Ho IC. GATA-3 regulates the development and function of invariant NKT cells. J Immunol. 2006;177:6650–6659.
23. Ku CJ, Hosoya T, Maillard I, Engel JD. GATA-3 regulates hematopoietic stem cell maintenance and cell cycle entry. Blood. 2012;119: 2242–2251.
24. Mjösberg J, Bernink J, Golebski K, Karrich JJ, Peters CP, Blom B, et al. The transcription factor GATA3 is essential for the function of human type 2 innate lymphoid cells. Immunity. 2012;37:649–659.
25. Hoyler T, Klose CS, Souabni A, Turqueti-Neves A, Pfeifer D, Rawlins EL, et al. The transcription factor GATA-3 controls cell fate and maintenance of type 2 innate lymphoid cells. Immunity. 2012;37:634–648.
26. Rothenberg EV. GATA-3 locks the door to the B-cell option. Blood. 2013;121:1673–1674.
27. Weaver CT, Hatton RD, Mangan PR, Harrington LE. IL-17 family cytokines and the expanding diversity of effector T cell lineages. Annu Rev Immunol. 2007;25:821–852.
28. Dong C. TH17 cells in development: an updated view of their molecular identity and genetic programming. Nat Rev Immunol. 2008;8:337–348.
29. Kaplan MH. Th9 cells: differentiation and disease. Immunol Rev. 2013;252:104–115.
30. Jöhrens K, Grünbaum M, Anagnostopoulos I. Differences in the T-bet and GATA-3 expression patterns between lymphocytic colitis and coeliac disease. Virchows Arch. 2010;457:451–456.
31. Holtmann MH, Neurath MF. T helper cell polarisation in coeliac disease: any (T-)bet? Gut. 2004;53:1065–1067.
32. Sapone A, Lammers KM, Mazzarella G, Mikhailenko I, Cartenì M, Casolaro V, et al. Differential mucosal IL-17 expression in two gliadin-induced disorders: gluten sensitivity and the autoimmune enteropathy celiac disease. Int Arch Allergy Immunol. 2010;152:75–80.
33. McAllister CS, Kagnoff MF. The immunopathogenesis of celiac disease reveals possible therapies beyond the gluten-free diet. Semin Immunopathol. 2012;34:581–600.
34. Harris KM, Fasano A, Mann DL. Monocytes differentiated with IL-15 support Th17 and Th1 responses to wheat gliadin: Implications for celiac disease. Clin Immunol. 2010;135:430–439.
35. Imperatore N, Rispo A, Capone P, Donetto S, De Palma GD, Gerbino N, et al. Gluten-free diet dose not influence the occurence and the Th1/Th17-Th2 nature of immune-mediated disease in patients with coeliac disease. Dig Liver Dis. 2016;48:740–744.
36. Liu H, Shi J, Wilkerson ML, Lin F. Immunohistochemical evaluation of GATA3 expression in tumors and normal tissues: a useful immunomarker for breast and urothelial carcinomas. Am J Clin Pathol. 2012;138:57–64.
37. Ordóñez NG. Value of GATA3 immunostaining in tumor diagnosis: a review. Adv Anat Pathol. 2013;20:352–360.
2. Kim SM, Mayassi T, Jabri B. Innate immunity: actuating the gears of celiac disease pathogenesis. Best Pract Res Clin Gastroenterol. 2015;29:425–435.
3. Villanacci V, Ceppa P, Tavani E, Vindigni C, Volta U; Gruppo Italiano Patologi Apparato Digerente (GIPAD), et al. Coeliac disease: the histology report. Dig Liver Dis. 2011;43:S385–S395.
4. Fernández Bañares F, Farré C, Carrasco A, Mariné M, Esteve M. New Tools for the Diagnosis of Celiac Disease. OmniaScience Monographs. 2015.
5. Mustalahti K, Catassi C, Reunanen A, Fabiani E, Heier M, McMillan S, et al. The prevalence of celiac disease in Europe: results of a centralized, international mass screening project. Ann Med. 2010;42:587–595.
6. Al-Hussaini A, Troncone R, Khormi M, AlTuraiki M, Alkhamis W, Alrajhi M, et al. Mass Screening for Celiac Disease Among School-aged Children: Toward Exploring Celiac Iceberg in Saudi Arabia. J Pediatr Gastroenterol Nutr. 2017;65:646–651.
7. Barada K, Bitar A, Mokadem MA, Hashash JG, Green P. Celiac disease in Middle Eastern and North African countries: a new burden? World J Gastroenterol. 2010;16:1449–1457.
8. Barada K, Abu Daya H, Rostami K, Catassi C. Celiac disease in the developing world. Gastrointest Endosc Clin N Am. 2012;22:773–796.
9. Bai JC, Fried M, Corazza GR, Schuppan D, Farthing M, Catassi C, et al. World Gastroenterology Organisation global guidelines on celiac disease. J Clin Gastroenterol. 2013;47:121–126.
10. Kurppa K, Ashorn M, Iltanen S, Koskinen LL, Saavalainen P, Koskinen O, et al. Celiac Disease Wthout Villous Atrophy in Children: A Prospective Study. J Pediatr. 2010;157:373–380.
11. Hudacko R, Kathy Zhou X, Yantiss RK. Immunohistochemical stains for CD3 and CD8 do not improve detection of gluten-sensitive enteropathy in duodenal biopsies. Mod Pathol. 2013;26:1241–1245.
12. Hayday A, Theodoridis E, Ramsburg E, Shires J. Intraepithelial lymphocytes: exploring the Third Way in immunology. Nat Immunol. 2001;2:997–1003.
13. Neutra MR, Mantis NJ, Kraehenbuhl JP. Collaboration of epithelial cells with organized mucosal lymphoid tissues. Nat Immunol. 2001;2:1004–1009.
14. Cheroutre H, Madakamutil L. Acquired and natural memory T cells join forces at the mucosal front line. Nat Rev Immunol. 2004;4:290–300.
15. Abadie V, Discepolo V, Jabri B. Intraepithelial lymphocytes in celiac disease immunopathology. Semin Immunopathol. 2012;34:551–566.
16. Meresse B, Malamut G, Cerf-Bensussan N. Celiac disease: an immunological jigsaw. Immunity. 2012;36:907–919.
17. Abadie V, Jabri B. Immunopathology of Celiac Disease. Mucosal Immunol (Fourth Edition). 2015;2:1551–1572.
18. Cheroutre H, Lambolez F, Mucida D. The light and dark sides of intestinal intraepithelial lymphocytes. Nat Rev Immunol. 2011;11:445–456.
19. Tahoun A, Mahajan S, Paxton E, Malterer G, Donaldson DS, Wang D, et al. Salmonella transforms follicle-associated epithelial cells into M cells to promote intestinal invasion. Cell Host Microbe. 2012;12:645–656.
20. Hoyler T, Connor CA, Kiss EA, Diefenbach A. T-bet and Gata3 in controlling type 1 and type 2 immunity mediated by innate lymphoid cells. Curr Opin Immunol. 2013;25:139–147.
21. Samson SI, Richard O, Tavian M, Ranson T, Vosshenrich CA, Colucci F, et al. GATA-3 promotes maturation, IFN-gamma production, and liver-specific homing of NK cells. Immunity. 2003;19:701–711.
22. Kim PJ, Pai SY, Brigl M, Besra GS, Gumperz J, Ho IC. GATA-3 regulates the development and function of invariant NKT cells. J Immunol. 2006;177:6650–6659.
23. Ku CJ, Hosoya T, Maillard I, Engel JD. GATA-3 regulates hematopoietic stem cell maintenance and cell cycle entry. Blood. 2012;119: 2242–2251.
24. Mjösberg J, Bernink J, Golebski K, Karrich JJ, Peters CP, Blom B, et al. The transcription factor GATA3 is essential for the function of human type 2 innate lymphoid cells. Immunity. 2012;37:649–659.
25. Hoyler T, Klose CS, Souabni A, Turqueti-Neves A, Pfeifer D, Rawlins EL, et al. The transcription factor GATA-3 controls cell fate and maintenance of type 2 innate lymphoid cells. Immunity. 2012;37:634–648.
26. Rothenberg EV. GATA-3 locks the door to the B-cell option. Blood. 2013;121:1673–1674.
27. Weaver CT, Hatton RD, Mangan PR, Harrington LE. IL-17 family cytokines and the expanding diversity of effector T cell lineages. Annu Rev Immunol. 2007;25:821–852.
28. Dong C. TH17 cells in development: an updated view of their molecular identity and genetic programming. Nat Rev Immunol. 2008;8:337–348.
29. Kaplan MH. Th9 cells: differentiation and disease. Immunol Rev. 2013;252:104–115.
30. Jöhrens K, Grünbaum M, Anagnostopoulos I. Differences in the T-bet and GATA-3 expression patterns between lymphocytic colitis and coeliac disease. Virchows Arch. 2010;457:451–456.
31. Holtmann MH, Neurath MF. T helper cell polarisation in coeliac disease: any (T-)bet? Gut. 2004;53:1065–1067.
32. Sapone A, Lammers KM, Mazzarella G, Mikhailenko I, Cartenì M, Casolaro V, et al. Differential mucosal IL-17 expression in two gliadin-induced disorders: gluten sensitivity and the autoimmune enteropathy celiac disease. Int Arch Allergy Immunol. 2010;152:75–80.
33. McAllister CS, Kagnoff MF. The immunopathogenesis of celiac disease reveals possible therapies beyond the gluten-free diet. Semin Immunopathol. 2012;34:581–600.
34. Harris KM, Fasano A, Mann DL. Monocytes differentiated with IL-15 support Th17 and Th1 responses to wheat gliadin: Implications for celiac disease. Clin Immunol. 2010;135:430–439.
35. Imperatore N, Rispo A, Capone P, Donetto S, De Palma GD, Gerbino N, et al. Gluten-free diet dose not influence the occurence and the Th1/Th17-Th2 nature of immune-mediated disease in patients with coeliac disease. Dig Liver Dis. 2016;48:740–744.
36. Liu H, Shi J, Wilkerson ML, Lin F. Immunohistochemical evaluation of GATA3 expression in tumors and normal tissues: a useful immunomarker for breast and urothelial carcinomas. Am J Clin Pathol. 2012;138:57–64.
37. Ordóñez NG. Value of GATA3 immunostaining in tumor diagnosis: a review. Adv Anat Pathol. 2013;20:352–360.
Downloads
Published
2018-09-15
How to Cite
1.
Omran TZ, Ahmed MM, Metib NJ. Assessment of GATA3 expression in duodenal biopsies of celiac disease suspected and diagnosed patients. Iraq Med J [Internet]. 2018 Sep. 15 [cited 2024 Nov. 23];2(3):72-4. Available from: https://iraqmedj.org/index.php/imj/article/view/442
Issue
Section
Articles