Diagnosis of Breast Cancer by some remarkable enzymes
Keywords:Breast cancer, remarkable enzymes
Objectives: This study aimed to evaluate the activity of several remarkable enzymes in the serum of breast cancer and estimate their potential value in the diagnosis of breast cancer.
Methods: The study comprised of 70 patients with breast cancer and 50 age and sex-matched healthy subjects as controls. Levels of (5 â€“Nucleotidase), Acid phosphatase activity (ACP), Glutamate dehydrogenase (GDH), Serum Î²-glucuronidase, Aldehyde dehydrogenase (ALDH), Alcohol Dehydrogenase (ADH), Superoxide dismutase (SOD) and Adenosine deaminase ADA were measured using enzymatic methods.
Results: There was a significant increase in the Catalytic activities of 5 â€“Nucleotidase((p<0.001), ACP ( p< 0.05), GDH ( â‰¤0.001 p), Serum Î²-glucuronidase (p<0.0001), ADH ( p< 0.001), SOD (p< 0.001))and ADA(p< 0.001),while there was no significant increase in the level of Aldehyde dehydrogenase (ALDH)( p = 0.231)in sera of breast cancer patients compared to the control.
Conclusion: The present study strongly indicates the potential role of SOD, GDH, ADH, GDH,5â€“Nucleotidase,GLU and ACP as important biomarkers in breast cancer.
 Genetic home reference, â€œBreast cancer,â€ 2015,
. Lynn JJ, Chen KF, Weng YM, Chiu TF. Risk factors associated with complications in patients with chemotherapy-induced febrile neutropenia in emergency department. Hematol Oncol., 2013; 31(4): 189â€“196.
. Raza, V.; Khanam, A.; Naimatullah, S. Evaluation of Non-Heamatological and Hepatic Toxicities Developed During Chemotherapy Treatment And Role of Liver Enzymes In Disease Prognosis. Journal of Biochemistry., 2010; 16(1): 10-16.
. Yalcin O, Karatas F, Erulas FA: The levels of glutathione peroxidase, vitamins A, E, C, and lipid peroxidation in patients with urothelial cÃ¡ncer. BJU Int., 2009; 93: 863-866.
. Liang SL, Chan DW: Enzymes and related proteins as cancer biomarkers: a proteomic approach. Clin Chim Acta., 2011; 381(1): 93-97.
. Durak I, Isik ACU, Canbolat O, Akyol O, Kavutcu M: Adenosine deaminase, 5 nucleotidase, xanthine oxidase, superoxide dismutase and catalase activities in cancerous and non-cancerous human laryngeal tissues.Free Radic Biol Med., 2010; 15: 681-4.
. Seth RK, Simmi Kharb, Kharb DP. Serum biochemical markers incarcinoma breast. Ind J of Med Sci., 2010; 257(8): 350-54.
. Stefanni M. Enzymes, isoenzymes ane enzyme variants in the diagnosis of cancer. A short review. Cancer., 2010; 55: 1931-6.
 Jemal A, Bray F, Center MM, Ferla J, Ward E, Forman D. Global cancer statistics. Cancer J Clin., 2011; 61: 69-90.
 Aghaei M, Tehrani FK, Salami S, Atri M. Adenosinedeaminase activity in the serum and malignant tumors of breast cancer: The assessment of isoenzymes ADA1 and ADA2 activities. Clin Biochem., 2010; 38(10): 887-91.
 Smita GN, Vaidya SM. Serum 5â€™adenosine deaminase,5â€™nucleotidase in breast carcinoma in stagewise manner.Journal of Advance Researches in Biological Sciences., 2013; 5(2): 135-8.
 L. Jin, D. Li, G. N. Alesi et al., â€œGlutamate dehydrogenase 1signals through antioxidant glutathione peroxidase 1 to regulate redox homeostasis and tumor growth,â€ Cancer Cell., 2015; 27(2): 257â€“270.
 G. Liu, J. Zhu, M. Yu et al., â€œGlutamate dehydrogenase is a novel prognostic marker and predicts metastases in colorectal cancer patients,â€ Journal of Translational Medicine., 2015. 13( 1): p. 144,
 D. M. Miller, S. D. Thomas, A. Islam, D. Muench, and K. Sedoris, â€œc-Myc and cancer metabolism,â€ Clinical Cancer Research., 2012; 18(20)
 B. J. Altman, Z. E. Stine, and C. V. Dang, â€œFrom Krebs to clinic: glutamine metabolism to cancer therapy,â€ Nature Reviews Cancer., 2016;16( 10): 619â€“634,
 Filmus JE, Podhajcer OL, Mareso E, Guman N, Mordoh J: Acid phosphatase in human breast cancer tissue. Cancer. 1984, 53: 301-305.
 Aanzerotti RH, Gullino PM: Activities and quantities of lysosomal enzymes during mammary tumor regression. Cancer Res. 1972, 32: 2679-2685.
 Gago-Dominguez M, Castelao JE, Pike MC, Sevanian A, Haile R. Role of lipid peroxidation in the epidemiology and prevention of breast cancer. Cancer Epidemiol Biomarkers Prev., 2005; 14:2829-39
 Yu B.P, Cellular defences against damage from reactive oxygen species. Biol Rev., 1994 74: 139 â€“ 163
 Jayaraman, K.S: Technology tradition unite Indiaâ€™s drug Discovery scheme. Nat. Med., 2003; 9: 982
 Antonyuk, S.V.; Strange, R.W.; Marklund, S.L. and Hasnain, S.S: The structure of human extracellular copper-zinc superoxide dismutase at 1.7 A resolution: insights into heparin and collagen binding. J. Mol. Biol., 2009:388 (2): 310â€“26
 Elchuri, S.; Oberley, T.D.; Qi, W.; Eisenstein, R.S.; Jackson, R.L.; Van, R. H.; Epstein C.J. and Huang, T.T: Cu Zn SOD deficiency leads to persistent and widespread oxidative damage and hepato carcinogenesis later in life. Oncogene., 2005;24 (3): 367â€“80
 Kaplan, J.H. and Groses, J.N: Liver and blood cell catalase activity of tumour bearing mice. Cancer Res., 1972; 26: 1190-94
 Å»Ã³Å‚taszek R., Hanausek M., KiliaÅ„ska Z.M., Walaszek Z.: The bioÂ¬logical role of D-glucaric acid and its derivatives: potential use in medicine. PostÄ™py Hig. Med. DoÅ›w., 2008; 62: 451-462
 Jelski et al. Alcohol Dehydrogenase (ADH) and Aldehyde Dehydrogenase (ALDH) as Candidates for Tumor Markers in Patients with Pancreatic Cancer. J Gastrointestin Liver Dis, 2011;20 ( 3): 255-259