Vitamin D3 Receptor Gene Polymorphism (rs 2228570) as a Predictor for Coronary Artery Diseases

Abstract

Objective: Present study aim to explore the association of vitamin D receptor VDR-FOKI gene polymorphism (rs2228570) with CAD in Iraqi population.

Methods: The current study case-control design included 300 samples (male and female) with age range between (40 – 70 year), 150 sample of them were randomly selected as normal healthy without any known disease as controls, whereas, another 150 cases of CAD subjects who underwent to the angiography department in the cardiology department of Al-Sadder medical city in Najaf governorate / Iraq and Al-Hussein Medical City /Holy Karbala - Iraq. Genotyping of rs2228570 polymorphism are carried out by PCR–RFLP. DNA was extracted from whole blood and genotyping was achieved with specific primers to amplify fragments. The enzyme FokI was used for the digestion of VDR gene product followed by electrophoresis on agarose gel.

Results: Genotype frequencies of rs2228570 polymorphism were found to be consistent with Hardy–Weinberg equilibrium. Allele frequencies of wild genotype TT (33.3%), heterozygous genotype TC(46.7%), and   homozygous genotype CC ( 20%) in cases of CAD group while 66.7%, 30 % and 3.3 % for  TT,  TC, and  CC  genotyping in the control group respectively. The genotype (CC) was significantly (OR= 7.25, 95% CI ; 2.74-19.20 , P<0.001( increased the risk of CAD seven and quarter folds with respect to those of (TT)  , while the TC genotype significantly (OR = 2.04, 95% CI  ;1.27-3.28,P<0.001)  raised the risk of CAD by two folds  .

Conclusion:   Our result improved that the gene polymorphism of VDR-FOKI was associated with high risk for development and progression of CAD  in  Iraqi population .

Keywords: Coronary artery disease (CAD); vitamin D receptor;   FokI