Evaluation of the relationship between Superoxide dismutase, Estradiol hormone, and the intracytoplasmic sperm injection outcome

Abstract

Objective: Multiple factors may be considered as a potential biomarker for Assisted Reproductive techniques success. The objective is to study the connection between superoxide dismutase as antioxidant and, estradiol hormone in patients undergoing assisted reproductive techniques and their effect on the intracytoplasmic sperm injection outcome.

Methods: Seventy women presenting with subfertility: male (35), unexplained (6), tubal (n = 4) and PCOS (n = 25). Blood samples were collected at oocyte retrieval. Blood was analyzed for estradiol hormone by using ELISA. Superoxide dismutase (SOD) was measured in serum by using a calorimetric method by adding serum to tris base and pyrogallole, immediately after addition of pyrogallole.

Result: There was insignificant difference between SOD, E2 in different cause (P>0.05). There was direct insignificant association between E₂ level and SOD (r = 0.151). There was direct correlation between serum Estradiol hormone and total oocyte retrieved, grade I embryos and total embryos (P<0.05). Also There was direct association between serum SOD and grade I embryos (P<0.01).

Conclusion(s): SOD considered as essential predictor of ICSI outcome. The direct relationship between E₂ and SOD suggests that Estradiol hormone may play a role in the ovarian metabolism.