Assessment of the Antibacterial Activity of Lavender cultivated in Tasmania and identifying its geographical and botanical origins
DOI:
https://doi.org/10.22317/imj.v7i1.1228Keywords:
Antibacterial Activity, Lavandula, TasmaniaAbstract
Objectives: This study compared the antimicrobial activity of lavender honey manufactured in Tasmania Australia with Manuka honey as a control.
Methods: Lavender essential oil also examined for antimicrobial activity. The volatile compounds were identified to find the bioactive compounds responsible for the antibacterial activity. Next, the volatile data of the Lavender honey and essential oil from Lavandula angustifolia cultivated in Tasmania, were used to indicate the geographical and botanical origins of the using head space solid-phase micro extraction (HS SPME) and Gas Chromatography Mass Spectrometry (GC-MS).
The antimicrobial activity of Lavender Honey and essential oil from Tasmania were examined using the broth micro-dilution method to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Bacteriostatic end points were determined spectrophotometrically, then bactericidal end points were determined by plating. Methicillin-sensitive and methicillin-resistant strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were used in this study.
Results: Lavender honey showed similar, and in some bacterial species, slightly higher activity than Manuka honey. The MICs and MBCs for lavender honey ranged from 6.25% to 25% v/v compared to MICs of Manuka honey which ranged between 12.5% and 25% v/v. The MIC and MBC values of lavender honey and Manuka honey against P. aeruginosa were 12.5% v/v and 25% v/v respectively. The corresponding values for a methicillin-sensitive strain of S. aureus were 6.25% v/v and 12.5% v/v respectively. The MIC and MBC were the same for both honey for E. coli equal to 12.5 % v/v and for MRSA equal to 6.25 % v/v. The growth of S. aureus, including MRSA and E. coli completely inhibited by Lavender E. oil at concentrations of 2.25%. Whereas, the growth of P. aeruginosa partly inhibited by the same concentrations. Use of a spectrophotometer facilitated reading of MIC values.
Conclusion: These results suggest that lavender honey and essential oil could be used as an antimicrobial agent for infections caused by S. aureus including MRSA, E. coli and P. aeruginosa.
References
Jamshidi-Kia F, Lorigooini Z, Amini-Khoei H. Medicinal plants: Past history and future perspective. Journal of herbmed pharmacology 2018;7(1):
Hammer KA, Carson CF, Riley TVJJoam. Antimicrobial activity of essential oils and other plant extracts. 1999;86(6):985-990
Joshi B, Sah GP, Basnet BB, et al. Phytochemical extraction and antimicrobial properties of different medicinal plants: Ocimum sanctum (Tulsi), Eugenia caryophyllata (Clove), Achyranthes bidentata (Datiwan) and Azadirachta indica (Neem). 2011;3(1):1-7
Bassolé IHN, Juliani HR. Essential Oils in Combination and Their Antimicrobial Properties. 2012;17(4):3989
Ouibrahim A, Tlili-Ait-kaki Y, Bennadja S, et al. Evaluation of antibacterial activity of Laurus nobilis L., Rosmarinus officinalis L. and Ocimum basilicum L. from Northeast of Algeria. 2013;7(4968-4973
Bais HP, Walker TS, Schweizer HP, et al. Root specific elicitation and antimicrobial activity of rosmarinic acid in hairy root cultures of Ocimum basilicum. 2002;40(11):983-995
Mandalari G, Bennett R, Bisignano G, et al. Antimicrobial activity of flavonoids extracted from bergamot (Citrus bergamia Risso) peel, a byproduct of the essential oil industry. 2007;103(6):2056-2064
Kaya I, Yigit N, Benli MJAJoT, Complementary, et al. Antimicrobial activity of various extracts of Ocimum basilicum L. and observation of the inhibition effect on bacterial cells by use of scanning electron microscopy. 2008;5(4):363-369
Basch E, Boon H, Heerema TD, et al. Boswellia: An evidence-based systematic review by the natural standard research collaboration. Journal of herbal pharmacotherapy 2004;4(3):63-83
Renata P, Krzysztof Bإ. Composition, biological properties and therapeutic effects of lavender L). A review. Herba Polonica 60(2):56-66, doi:doi:10.2478/hepo-2014-0010
Ciocarlan A, Lupascu L, Aricu A, et al. Chemical composition and assessment of antimicrobial activity of lavender essential oil and some by-products. Plants 2021;10(9):1829
Henriques AF, Jenkins RE, Burton NF, et al. The effect of manuka honey on the structure of Pseudomonas aeruginosa. European journal of clinical microbiology & infectious diseases 30(167-171
Molan PC. The antibacterial activity of honey: 2. Variation in the potency of the antibacterial activity. Bee World 1992;73(2):59-76
Ohashi K, Natori S, Kubo T. Expression of amylase and glucose oxidase in the hypopharyngeal gland with an age-dependent role change of the worker honeybee (Apis mellifera L.). European Journal of Biochemistry 1999;265(1):127-133, doi:10.1046/j.1432-1327.1999.00696.x
Mandal MD, Mandal S. Honey: its medicinal property and antibacterial activity. Asian Pacific journal of tropical biomedicine 2011;1(2):154-160
Brudzynski K, Abubaker K, Miotto D. Unraveling a mechanism of honey antibacterial action: Polyphenol/H 2O 2-induced oxidative effect on bacterial cell growth and on DNA degradation. Food Chemistry 2012;133(2):329-336, doi:10.1016/j.foodchem.2012.01.035
Kwakman PH, Zaat SA. Antibacterial components of honey. IUBMB life 2012;64(1):48-55
Stanimirova I, Üstün B, Cajka T, et al. Tracing the geographical origin of honeys based on volatile compounds profiles assessment using pattern recognition techniques. Food Chemistry 2010;118(1):171-176, doi:10.1016/j.foodchem.2009.04.079
Yamani H. Production of bioactive honey from medicinal plants using alternative and conventional production methods. RMIT University: Melbourne; 2015.
Yamani HA, Pang EC, Mantri N, et al. Antimicrobial activity of Tulsi (Ocimum tenuiflorum) essential oil and their major constituents against three species of bacteria. Frontiers in Microbiology 2016;7(doi:https://doi.org/10.3389/fmicb.2016.00681
Da Porto C, Decorti D. Analysis of the volatile compounds of flowers and essential oils from Lavandula angustifolia cultivated in Northeastern Italy by headspace solid-phase microextraction coupled to gas chromatography-mass spectrometry. Planta Medica 2008;74(02):182-187
Mandal S, DebMandal M, Pal NK, et al. Synergistic anti–Staphylococcus aureus activity of amoxicillin in combination with Emblica officinalis and Nymphae odorata extracts. Asian Pacific Journal of Tropical Medicine 2010;3(9):711-714
Mandal S, DebMandal M, Pal NK, et al. Antibacterial activity of honey against clinical isolates of Escherichia coli, Pseudomonas aeruginosa and Salmonella enterica serovar Typhi. Asian Pacific Journal of Tropical Medicine 2010;3(12):961-964
Cavanagh HMA, Wilkinson JM. Biological activities of lavender essential oil. Phytotherapy Research 2002;16(4):301-308
Lis-Balchin M, Deans SG, Eaglesham E. Relationship between bioactivity and chemical composition of commercial essential oils. Flavour and Fragrance Journal 1998;13(2):98-104
Pattnaik S, Subramanyam VR, Bapaji M, et al. Antibacterial and antifungal activity of aromatic constituents of essential oils. Microbios 1997;89(358):39-46
Willix DJ, Molan PC, Harfoot CG. A comparison of the sensitivity of woundâ€گinfecting species of bacteria to the antibacterial activity of manuka honey and other honey. Journal of applied bacteriology 1992;73(5):388-394
Molan PC. Potential of honey in the treatment of wounds and burns. American Journal of Clinical Dermatology 2001;2(1):13-19
Bogdanov S. Nature and Origin of the Antibacterial Substances in Honey. LWT - Food Science and Technology 1997;30(7):748-753, doi:http://dx.doi.org/10.1006/fstl.1997.0259
Adams CJ, Boult CH, Deadman BJ, et al. Isolation by HPLC and characterisation of the bioactive fraction of New Zealand manuka (Leptospermum scoparium) honey. Carbohydrate research 2008;343(4):651-9, doi:10.1016/j.carres.2007.12.011
Henriques AF, Jenkins RE, Burton NF, et al. The effect of manuka honey on the structure of Pseudomonas aeruginosa. European Journal of Clinical Microbiology & Infectious Diseases 2011;30(2):167-171, doi:10.1007/s10096-010-1065-1
Louveaux J, Maurizio A, Vorwohl G. Methods of melissopalynology. Bee world 1978;59(4):139-157
La Serna-Ramos I. Las determinaciones melisopalinolأ³gicas en la tipificaciأ³n de la miel y su aplicaciأ³n al control de calidad. 1a Jornadas de la Miel de Canarias: 2007.
Saenz C, Prada C, Gأ³mez Ferreras C, et al. Tأ©cnicas de Palinologأa actual. Anuario APLE 1980;1980(16-26
Castro-Vأ،zquez L, Leon-Ruiz V, Alaأ±on ME, et al. Floral origin markers for authenticating Lavandin honey (Lavandula angustifolia x latifolia). Discrimination from Lavender honey (Lavandula latifolia). Food Control 37(362-370
Guyot-Declerck C, Renson S, Bouseta A, et al. Floral quality and discrimination of Lavandula stoechas, Lavandula angustifolia, and Lavandula angustifoliaأ— latifolia honeys. Food Chemistry 2002;79(4):453-459
Bouseta A, Collin S, Dufour J-P. Characteristic aroma profiles of unifloral honeys obtained with a dynamic headspace GC-MS system. Journal of Apicultural Research 1992;31(2):96-109
Bouseta A, Scheirman V, Collin S. Flavor and free amino acid composition of lavender and eucalyptus honeys. Journal of Food Science 1996;61(4):683-687